Roles for transcript leaders in translation and mRNA decay revealed by transcript leader sequencing
نویسندگان
چکیده
منابع مشابه
Roles for transcript leaders in translation and mRNA decay revealed by transcript leader sequencing.
Transcript leaders (TLs) can have profound effects on mRNA translation and stability. To map TL boundaries genome-wide, we developed TL-sequencing (TL-seq), a technique combining enzymatic capture of m(7)G-capped mRNA 5' ends with high-throughput sequencing. TL-seq identified mRNA start sites for the majority of yeast genes and revealed many examples of intragenic TL heterogeneity. Surprisingly...
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ژورنال
عنوان ژورنال: Genome Research
سال: 2013
ISSN: 1088-9051
DOI: 10.1101/gr.150342.112